Composite

Part:BBa_K4890013:Design

Designed by: Duoqing LIN   Group: iGEM23_Shanghai-SDG   (2023-08-14)


UAS-Hsp70-MTF1


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 382
    Illegal EcoRI site found at 2296
    Illegal PstI site found at 244
    Illegal PstI site found at 2054
    Illegal PstI site found at 2232
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 382
    Illegal EcoRI site found at 2296
    Illegal NheI site found at 509
    Illegal PstI site found at 244
    Illegal PstI site found at 2054
    Illegal PstI site found at 2232
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 382
    Illegal EcoRI site found at 2296
    Illegal BglII site found at 527
    Illegal BamHI site found at 479
    Illegal BamHI site found at 1468
    Illegal BamHI site found at 1864
    Illegal BamHI site found at 1891
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 382
    Illegal EcoRI site found at 2296
    Illegal PstI site found at 244
    Illegal PstI site found at 2054
    Illegal PstI site found at 2232
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 382
    Illegal EcoRI site found at 2296
    Illegal PstI site found at 244
    Illegal PstI site found at 2054
    Illegal PstI site found at 2232
    Illegal NgoMIV site found at 1730
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Our team aims to detect the amounts of heavy metals by transgenic fruit flies. We plan to insert MTF-1 gene driven by UAS and Hid gene driven by MRE in Drosophila embryos, S2 cells, respectively. These two transgenic Drosophila lines interbreed and the offspring with both MTF-1 and Hid genes cross with Drosophila with genotype GMR-GAL4 or Vg-GAL4 or ptc-GAL4. In this way, we constructed three Drosophila lines with genotype UAS-MTF-1;MRE-Hid/GMR-GAL4 , UAS-MTF-1;MRE-Hid/Vg-GAL4 and UAS-MTF-1;MRE-Hid/ptc-GAL4. All these lines can be used as a visual monitoring system for heavy metals. In response to different heavy metal concentrations in the growing environment, they show observable abnormally small eye/wing sizes in the first generation within 5-10 days.


Source

UAS sequence is based on BBa_K3281012 Hsp70 sequence is based on BBa_K4890004 MTF-1 gene is based on BBa_K4890001


References